Considering that 5-azacytidine inhibits NMD of a chimeric PTC reporter gene construct, we next tested whether it also modulates the expression of the so-called endogenous NMD targets (Mendell et al, 2004).
Having established similar expression levels in cells transfected with Rev-dependent or PRE-dependent Gag constructs, we next sought to compare the extracellular VLP production between cells transfected with either the Rev-dependent or PRE-dependent HIV-1 Gag.
Having established the activity of the different Nek8 constructs, we next examined their localization, specifically in terms of their ability to localize to centrosomes in dividing cells and cilia in serum-starved quiescent cells.
Next, using the Ulp1(3)constructstruct, we show that the septin-targeting information is limited to region 3 of Ulp1.
We next constructed a luciferase construct with the GRN 3′-UTR sequence in which the complete 18 bp predicted binding site of miR-659 was deleted (position 71 88 downstream of the termination codon; vectorΔ18) (Fig. 4C).
To acquire the braid index, we first constructed the overlap graph G o(S) of S. We next constructed a simplified string graph G s(S) of S which is obtained from G o(S) by removing contained reads, transitive edges, and concatenating, "one-in one-out" nodes.
We constructed a next-generation optical trapping instrument to study the motility of single motor proteins, such as kinesin moving along a microtubule.
We next constructed a CRAd (Ad-κBF512HRE) where E1A activity was under κBF512HRE regulation.
We next constructed biosensors for detection of protein kinase A (PKA) and c-Jun N-terminal kinase (JNK) activities29.
We next constructed an in vitro kinase assay of purified recombinant RAF1, HSP90β, and CDC37, and demonstrated that KBU2046 decreased phosphorylation of RAF1's Ser338 activation motif (Fig. 6e).
