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The primary tools that are being used to execute genome excision are constructed using zinc fingers (ZF) (3) and transcription activator-like effector (TALE) (4) proteins but they have their own limitations.
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In the present study, to provide a basis for designing ATFs that can regulate the human A20 gene, a ZFP sequence involving six contiguous Cys2His2-type zinc fingers was constructed using the classical Cys2His2-type zinc finger model and an 18 bp DNA sequence (as target sequence) obtained from the A20 gene promoter region.
The huts are constructed using plastic ropes, heavy rocks, wood, bamboo, zinc plates and iron rods.
Phylogenetic trees were constructed using the MEGA5 package69.
The genome maps were constructed using GCView57.
Kymographs were constructed using Metamorph 7.1.4 software.
Neighbor-joining trees were constructed using NEIGHBOR.
Phylogenetic trees were constructed using MEGA3 [73].
Figures and movies were constructed using CHIMERA [98].
Strains were constructed using standard genetic techniques.
Maps were constructed using maps2WinBUGS version 1.6.
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