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The structures were constructed using the program COOT40 and refined using the program REFMAC541,42 with the Translation Libration Screw refinement technique.
Input files for various software were constructed using the program Create when possible [26].
Models based on each template were constructed using the program NEST [29].
Median joining networks were constructed using the program NETWORK, ver. 4.1.1.2 [66].
MJN of the S-gene quasispecies of HBV/E and HBV/A3 were constructed using the program NETWORK 4.0 [26].
The phylogenetic tree was constructed using the program protml.exe of the package Phylip v. 3.65 [56], [56].
Similar(27)
Specifically, 100 bootstrap replicates of the alignment were constructed using the SEQBOOT program, and the distances between sequences were computed by the PROTDIST program using the PAM substitution model.
The JTT distance matrix of protein sequences was constructed using the PROTDIST program and the neighbour-joining phylogenetic tree was constructed using the NEIGHBOR program of the Phylip package [ 23].
Phylogenetic trees were constructed using the CLUSTAL_X program following alignment of sequences by neighbor-joining algorithms [ 28] and visualized using the TreeView program [ 24].
The maximum parsimony trees were constructed using the PROTPARS program.
Starting phylogenetic trees were constructed using the modified program BIONJ.
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CEO of Professional Science Editing for Scientists @ prosciediting.com