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It should be noted that PBPK models constructed using in silico Kp parameters (i.e., without measured tissue data) may represent an oversimplification of tissue kinetics.
To accomplish this, a DNA microarray comprised of over 41000 oligonucleotides was designed and constructed using in situ inkjet oligonucleotide synthesis.
Plasmid pCCBX-YR was constructed using in vivo recombination in the yeast S. cerevisiae DS94 (MATα, ura3-52, trp1-1, leu2-3, his3-111, and lys2-801 (Tang et al., 1996) following published procedures (Raymond et al., 1999).
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Primers and probes were constructed using in-house software.
The networks were constructed using in-house Perl scripts.
Percentages of nucleotide identity were calculated from alignments performed with MUMer v3.0 [ 67] and genome maps were constructed using in-house scripts.
To patent a novel gene construct used in this or that creature seems fair enough, as it is truly inventive.
The Beclin 1 promoter construct used in our study comprises 949 bp (nucleotide position −856/+93).
The luciferase reporter construct used in one-hybrid analysis has been described elsewhere [15].
The mVenus:mCerulean construct used in Figure 6B is the short linker sensor previously described [20].
This is in contrast to the dnMSTAT construct used in this study, which simply required translation and secretion.
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CEO of Professional Science Editing for Scientists @ prosciediting.com