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The calibration curve was constructed using glycine as standard [ 13].
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A BAC library was constructed using HindIII for Glycine soja PI468916, called GSS_Ba.
The models were constructed using the OI triple helical glycine substitutions mutations published by the OI consortium (5) as a training set.
An un-rooted tree was constructed using the mature sequences of miR1511 among the subgenus Glycine, and two as outgroup which were not in stem region using the maximum likelihood (ML) method.
In case of glycine, a representative of the Cβ position gets constructed using the backbone as anchor.
Kinase Assays GST-MEF2tide-WT and GST-MEF2tide-T20A fusion protein constructs encoding GST, a glycine-rich linker and amino acids 14-24 of MEF2 were constructed using the Gibson Assembly Kit (NEB) in pGEX-KG and confirmed by sequencing.
Phylogenetic trees were constructed using the MEGA5 package69.
The genome maps were constructed using GCView57.
Phylogenetic trees were constructed using MEGA3 [73].
Kymographs were constructed using Metamorph 7.1.4 software.
Neighbor-joining trees were constructed using NEIGHBOR.
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