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An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.

An index of these household assets was constructed using an established principal component analysis method [ 17] to weight the contribution of each asset to the index.

Data files are constructed using an ASCII-type editor (see examples below and in subdirectory DATA).

The effector vectors without MS2 coat protein were constructed using an RT-PCR and In-Fusion cloning method (Takara 9.

In order to perform this optimisation, a mathematical model is constructed using an iterative experimental design.

Dynamic spanning trees (DST) are constructed using an ARMA FIEGARCH cDCC process.

Then a fuzzy inference system (FIS) model for slip estimation is constructed using an adaptive neuro-fuzzy inference system (ANFIS).

Bifurcation diagrams are constructed using an efficient pseudo-arc-length continuation method coupled with a powerful Newton Krylov-subspace solver.

Digital movies were constructed using an automated MATLAB routine (developed in Version 7.11.1.866 (R2010b) service pack 1) for drift correction in x, y and z dimension, respectively, and Fiji (ImageJ 1.51 g) (National Institute of Health, USA).

Photo-electrochemical cells were constructed using an illuminated rutile crystal as the anode and several another counter electrodes as the cathode.

The aluminum pyramidal cores were constructed using an interlocking method before curing with composite face sheets to fabricate the final structure.

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