As we construct only the transformations that can superimpose at least two physico-chemically similar interactions, we reduce the number of the constructed transformations and achieve a performance gain over other methods (e.g. MultiBind, see Table 1 in Additional file 2).
The binary vector pBI121 (EMD Bioscience Inc., Gibbstown, NJ, USA), which contains the NPT II gene encoding kanamycin resistance, 35S and uidA, was used to construct transformation units.
In this paper we describe how we can use a library, which was designed for constructing transformations of typed abstract syntax, in the removal of left recursion from a typed grammar description.
In total, four transformation vectors, designated pLSP15 (PHB) and pLSP20 (PHBV), and pLSP13 (PHB) and pLSP23 (PHBV), were constructed for transformation in Arabidopsis thaliana and oil palm, respectively.
Yeast strain AACK-GCS1 was constructed by transformation of the "UP GCS1 -amdSYM GCS1 -TEF GCS1 -DW GCS1 UP GCS1 -amdSYM GCS1 -TEF GCS1 -DW GCS1 UP GCS1 -amdSYM GCS1 -TEF GCS1 -DW GCS1 UP GCS1 -amdSYM GCS1 -TEF GCS1 -DW GCS1
Integration plasmids were constructed for transformation with up to three copies of the crtYB gene.
TMB4132 and TMB4133 were constructed by transformation of CEN.PK2-1C with expression cassettes for TRP1 and LEU2 or the GPD1 (TRP1) and GPD2 (LEU2) deletions cassettes.
The strains AACK-GLO3, YIGLO3, GLO3 + EG and GLO3 + AGL were constructed by transformation of the "UP GLO3 -amdSYM GLO3 -TEF GLO3 -DW GLO3 UP GLO3 -amdSYM GLO3 -TEF GLO3 -DW GLO3 UP GLO3 -amdSYM GLO3 -TEF GLO3 -DW GLO3 UP GLO3 -amdSYM GLO3 -TEF GLO3 -DW GLO3
Kiguradze constructed a transformation that reduces a second-order Emden-Fowler equation to an equation with almost constant coefficients and developed a technique for the study of the latter.
In the previous study (Kato et al. 1997), by employing H. fabianii J640 u-1 as a host strain and pHFura3 as a vector plasmid, we constructed a transformation system of H. fabianii J640.
