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The story of the life they have constructed together is told in "Oma & Bella," a documentary by Alexa Karolinski, a 28-year-old German-Canadian filmmaker who was born and raised in Berlin.
In the whimsical Norwegian film "Tuba Atlantic," directed by Hallvar Witzo, a 70-year-old man who is given six days to live tries to contact his estranged brother in America with the world's largest tuba, which they had constructed together as children.
The practice field would be constructed together by multiple players who have competition and coordination relationships.
At KIT, a pilot plant has been constructed together with industrial partners to demonstrate the process chain in representative scale.
From here, the physical-laws and geological concepts were constructed together with the teachers, making always the paragon with Māori knowledge.
A Social Accounting Matrix SAMM) was constructed together with energy consumption with CO2 emission were compiled, and then calibrated with a computable general equilibrium (CGE) model.
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We tested the importance of this interaction by co-expressing a dominant negative Rac3 construct together with the ABD of βI-spectrin.
Cells were then transfected with 250 ng/well of a 3×κB luciferase reporter construct together with 2.5 ng/well of pRL-TK Renilla luciferase construct (Promega, Madison, WI) using 2 µg polyethylenimine/µg of DNA.
MSCs were co-transfected with the –1682pGL3 construct together with either an empty pMSCV or a pMSCV construct containing sequences encoding the EWS-FLI-1, EWS-ERG or FUS-ERG fusion.
Co-transfection of this construct together with an expression vector for MYCN in SH-EP cells led to a sharp transactivation of the luciferase activity, which was not detected in the control empty vector group (Figure 1E).
To confirm the role of NFAT on cell death induced by TNF-α, we cotransfected the NFAT promoter construct together with a plasmid encoding a version of NFAT (dnNFAT) that lacks the trans activation domain, and acts as dominant negative.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com