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Mutant ΔF508/V510R/R1070D was constructed to reverse the positions of the charged residues.
A final expression plasmid was constructed to reverse the orientation of the His tag of fusion protein α2AP(13-73)-HSAH6 [ 19].
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We observed that TatA-Y1 and TatA-Y2 are able to form functional translocases with endogenous TatB and TatC as evidenced by the ability of these constructs to reverse the chain phenotype of ΔtatAE cells (Fig. 4b).
RNA templates carrying mutations in the U5-IR stem and loop were constructed to examine reverse transcription in vitro.
Two SSH libraries, forward and reverse, were constructed to identify genes up- and down-regulated in R. microplus male salivary glands in response to A. marginale infection.
Those charred pages are still kept at the University of Pennsylvania in Forrest's case, "perhaps the only book," the show tells us, "whose remains are preserved in a specially constructed sarcophagus" — or, to reverse Jonson's tribute, creating a tomb without a monument.
The forward SSH libraries were constructed to be enriched for genes that were up-regulated by heat-shock, and the reverse SSH libraries were constructed to be enriched for genes that were down-regulated by heat-shock.
Nevertheless, stable introduction of the R-Yes construct was sufficient to reverse the clustering phenotype of c-Yes knock-down cells, (Fig. 4B).
In this study, transfection with BRCA1 anti-sense constructs was shown to reverse both processes [ 44].
This study demonstrates the feasibility of using constructed wetlands treated with coagulants to reverse subsidence by accreting the resulting organo-metal flocculent and storing carbon at rates exceeding untreated wetlands.
Thus, combining IL-18 cDNA with OVA cDNA resulted in a vaccine construct that protected against the development of AHR, and that was unique among cDNA constructs in its capacity to reverse established AHR.
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