Sentence examples for constructed to replace from inspiring English sources

To place another loxP site at the Gabrb3 locus, another replacement-type targeting vector of Gabrb3-pG12 was constructed to replace part of exon 1 and exon 2 of Gabrb3 with a cassette containing a loxP site, a puromycin selectable marker, and a 3'-half of the Hprt exon 3 to 9 minigene.

A brick house was constructed to replace their shack of mud and dung.

In the 18th century, fires destroyed large parts of the city, and stone buildings were constructed to replace the old wooden houses.

An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives.

In OLVFM-MOGA, the variable-fidelity metamodel (VFM) is constructed to replace the expensive simulation models to ease the computational burden.

Additionally, an inactive PRK mutant PRK2021, which carries K20M and S21A mutations in the conserved nucleotide-binding sites of ATP-binding proteins (Higgins et al., 1986), was constructed to replace the wildtype gene in the selective plasmid.

The first allele of TbAGO1 was replaced by the BSD marker before the obtained cell line was transfected with the NEO construct to replace the second allele.

Modifying the construct to replace a lysine residue with a cysteine was performed to provide greater selectivity in the presence of basic CPPs.

Resistant cell lines were obtained at the expected frequency and further transformed with the NEO construct to replace the second TbAGO1 allele.

The BbvCI/ ApaI fragment containing the promoter deletion was then liberated from each deletion construct to replace the corresponding fragment in PCRA-10kb-tag to generate PCRA-10kb-tag-214 and PCRA-10kb-tag-1226.

Regenerative medicine approaches facilitate the use of biological constructs to replace or regenerate normal tissue function.