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Recombinant Ad genomes were constructed to encode a CMV promoter/firefly luciferase expression cassette in the E1 region, the 566FF-R-R34 or 566FF-EE12RR345L fiber and the complementary zipper-scFv G28-5 intorthe intacthE3intact E3 region in the place of the deleted 19 kDa protein (Fig. 1D).
Therefore, a 4-digit binary signature was constructed to encode the presence or absence of species aquaporin expression.
The chimeric VSV/SAD G VSV GRtmCcDNADNA was constructed to encode the ectodomain (aa 1 454) of VSV Indiana G (kindly provided by Dr. John K. Rose) fused to the entire transmembrane and cytoplasmic domain (amino acids 450 524) (Mebatsion et al. 1995) of RABV SAD G.
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The last exon of IAA28 has been modified in this construct to encode five copies of the c-Myc epitope immediately upstream of the IAA28 termination codon.
Further, BioPAX Level 3 provides very interesting constructs to encode several instances of the same protein.
DNA vaccines are bacterial plasmids constructed to express an encoded protein following in vivo administration and their subsequent uptake by cells.
A construct engineered to encode HsNPC1 domain C (residues 372 622) flanked by sequences that form a stable, antiparallel coiled coil, and fused to a preprotrypsin signal sequence with flag and hexahistidine tags at its N terminus has been described (Deffieu and Pfeffer, 2011; Miller et al., 2012).
In this study, EGSs were constructed to target the mRNA encoding human CC-chemokine receptor 5 (CCR5), one of the primary coreceptors for HIV.
All Ire1 variants were constructed to contain a C-terminally encoded HA-tag.
The marker plasmid pAH was constructed to express the hygromycin resistance-encoding gene (hpt) under the control of a constitutive promoter (for a gene encoding a cytosolic actin) (Fig. 1a).
Multiple transgenic Drosophila strains were also generated using constructs designed to encode hApp and hApp+1 proteins (Supplemental materials).
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Justyna Jupowicz-Kozak
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