The ERα mRNA copy numbers in tumor tissues were determined in comparison with a standard graph constructed simultaneously using 10, 10, 10, 10, 10, 10, 10, and 109 copies of reverse transcribed cRNA of ERα.
Multivariate ANOVA models for each cognitive change score were constructed, simultaneously including APOE genotype and any risk factors found to predict cognitive decline in association with genotype.
Lysates from HEK 293T cells transfected either with an empty vector as a control or with Ack1, TrkA or with both constructs simultaneously, and immunoprecipitated with anti-HA or with anti-TrkA antibody revealed TrkA after immunoprecipitation with anti-HA (upper panel, fourth lane) as well as Ack1 after Trk immunoprecipitation (third panel, fourth lane).
We anticipated that the variability of luciferase-based assays may be due to the requirement for transfection with multiple constructs simultaneously.
Linear models were constructed to simultaneously estimate fixed, polygenic and QTL effects.
SW1353 chondrosarcoma cells were transfected with CAGA and Renilla [65] constructs simultaneously (Renilla is used to control for transfection efficiency) using Fugene 6 (Sigma Aldrich, Dorset, UK).
In the experiments utilizing bone marrow from Ink4bKORbfl/fl mice, Lin− cells were infected with two viral constructs (MIG- and Lenti-X-pTuner-based constructs) simultaneously using a mixture of highly concentrated viral supernatant (>10 infectious unit/ml each).
All factors were used as predictors of all constructs simultaneously.
Let t1, t2, ⋯, t8 be the known branch lengths (estimated simultaneously with constructing the species tree), y6, y7 and y8 be the unknown gene family sizes of the three internal ancestral nodes of the tree, and let y0 be the gene family size at the LUCA, each of y i taking values from {0,1, m}.
Two multivariable models were constructed with all variables entered simultaneously to generate adjusted odds ratios (AORs) and 95% CIs with p < .05 defining statistically significant factors.
