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To search these databases, we constructed profile hidden Markov models (HMMs) of each MST subfamily.
The 6-frame translatiof of the Tasmanian devil genome was then searched using the constructed profile HMM using HMMER v3.0.
The 6-frame translatiof of koala transcriptomes were then searched using the constructed profile HMM using HMMER v3.0 [ 25].
Therefore, we constructed profile HMMs for each individual subunit, using all available sequences clustered at various identity thresholds.
Each genome encoding the studied transcription factor was scanned with the constructed profile using the GenomeExplorer software [ 37], and genes with candidate regulatory sites in the upstream regions were selected [ 38].
In particular, the performance of PSSMs converted from HMMs (PO) versus those iteratively constructed (PC and PS) shows that a more carefully constructed profile may yield better performance, with the difference being more pronounced in SUPERFAMILY than in Pfam.
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Month after month, the gullible and self-important celebrities of the day – Jeremy Beadle, Jeffrey Archer, Robert Maxwell, Samantha Fox, Keith Floyd, Bernard Manning, David Mellor, Sir Jimmy Savile – would find their pearls of wisdom gently lampooned and their carefully constructed profiles vigorously barbecued.
The BKA has constructed profiles of 60 radical Islamists.
Accordingly, phase stability profiles were constructed between 0 and 20 Hz in 0.1 Hz increments, applying 1 Hz smoothing regularization, for each stimulation block, and compared with analogously constructed profiles for the sham stimulation condition.
Similarly, we constructed profiles for the presence of the twin-arginine transport (TAT) signal sequence (TIGR01409) and the type IV pilin-like N-terminal cleavage and methylation signal (TIGR02532).
We used our set of maize (12 'seed' helitrons and 23 'predicted' helitrons) and similarly identified rice helitrons to construct profile HMMs for each terminal region and each species.
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