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KM constructed phage libraries.
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Using G2 as a scaffold, we constructed phage-displayed libraries of FHA1 variants and affinity selected for variants that bound selectively to five pT peptides.
In this study, establishment of nano-bioprobes was demonstrated, based on the T7 phage display system, by constructing phage particles displaying a ligand polypeptide S-tag and a green fluorescent protein (GFP) at the same time on the surface of phage head.
Additionally, unique restriction sites can be introduced to create mycobacteriophage cloning vectors and for constructing phage chimeras.
For each transposon construct, phage lysate was mixed with 25 ml recipient strain in SGMM (multiplicity of infection = 5).
mRNA extracted from lichen thallus and axenically grown fungal and algal symbionts were used to construct phage cDNA libraries using the ZAP-cDNA Gigapack III Gold Cloning (#200450) cDNA library synthesis kit (Stratagene, La Jolla, USA) according to manufacturer's instructions.
Importantly, both single and double Avd and Avd(N118M) construct phages were competed against each other.
A comparative phage proteomic tree was constructed using Phage Proteomic Tree version 4 (PPT, http://phage.sdsu.edu/rob/PhageTree/v4).sdsu.edu/rob/PhageTree/v4
MG1655 containing the rpsAD210Y mutation and WC196LC containing the rpsAD210Y mutation were constructed by phage P1 transduction using the phage P1 obtained from the FitnessOle ycaI deletion strain.
The goal of phage strain construction is to construct isogenic phage strains that differ in two traits: adsorption rate and marker state.
A nanobody library of 8 × 108 clones was constructed and phage displayed.
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