Sentence examples for constructed lacking from inspiring English sources

To prevent the import of the three enzymes into yeast mitochondria, N-terminally shortened Ilv2, Ilv5 and Ilv3 versions were constructed lacking their mitochondrial targeting sequences.

To further confirm active secretion and to rule out the possibility of cell lysis as a means of generating CipA* in the supernatant, a second strain was constructed lacking the predicted native sec tag [ 35] (Additional file 1: Figure S2) and the localization of cipA* in the two strains was compared.

To this end, a Cd146 construct lacking the KKGK motif (coding Cd146-∆KKGK) or a Moesin construct lacking the actin-binding domain (coding Moesin-∆ABD) was transfected into 10T1/2 cells.

The DNA vaccine construct lacking both the SS and the TD domain and the vaccine having only the SS generated lower antibody responses, compared to the rGVRt construct.

We further show that an ND1 construct lacking D2 but including the linker between D1 and D2 is catalytically active, resolving a conflict in the literature.

The luciferase construct lacking the PPRE and the Renilla constructs were purchased from Promega.

A construct lacking all 8 amino acids was unable to rescue the tre1sctt defect (Figure 3).

This procedure produced a WD construct lacking part of the F-box and conjugated to FLAG.

Inhibitory potency was the same for a prodomain construct lacking only the upstream cytosolic and transmembrane domains (Tyr54-Asp243) and for constructs lacking the upstream 104 (Ser126-Asp243), Leu127-Asp243sp243), or 154 (Leu155-amino3) acids acids of the prodomain (Fig. 2).

The decreased phosphorylation of AKT deletion construct lacking PH domain could be due to poor binding with activated Ack1.

In agreement with previous studies, the Head1/Neck3 construct (lacking the conventional neck domain) shows clear defects in processivity.