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* Libraries 2 to 6 were constructed from wheat cv Norstar.
We generated 73,521 quality-filtered ESTs from eleven cDNA libraries constructed from wheat plants exposed to various abiotic stresses and at different developmental stages.
For testing the applicability of PstI/ TaqI method of complexity reduction for the DArT analysis of the rye genome, 12288 clones from a diversity panel constructed from wheat DNA [[ 16], http://www.diversityarrays.com] were printed onto microarrays.
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To obtain insights into the molecular basis of the compatible interaction between wheat and Pst, we report here an EST library constructed from fully susceptible wheat leaves infected with a highly virulent pathotype of Pst.
Recently, an EST library was constructed from fully susceptible wheat leaves inoculated with P. graminis f.sp.sp
This is probably due to that the small RNA library for 454 sequencing was constructed from the pooled wheat RNA isolated from leaves, roots and spikes.
Sequencing and characterizing of a cDNA library constructed from Fg infected wheat spikes did enrich the understanding of gene expression profiling.
Eighteen libraries from various growth stages, 25 libraries from abiotic stresses (cold, drought, saline, and mineral toxic), and eight libraries from biotic stresses (leaf rust, powdery mildew, and blast) were constructed from eight different wheat lines (Table 1).
Maq-120 alignment was also performed on the component of reads that failed to map to the NCBI Unigene reference against a new reference constructed from a 5× assembly of wheat Chinese Spring genomic 454 reads (M. Bevan, JIC, personal communication).
Wheat cDNA libraries were constructed from poly(A)+ mRNA from 3 5 mm Triticum aestivum (L. cv.
Wax platelets, characteristic for wheat leaves (Triticum aestivum), are constructed from the primary alcohol octacosan-1-ol [28].
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