A year and a half ago he decided to build a second observatory, a dome he constructed from a $10,000 kit made by an outfit called Technical Innovations, in Gaithersburg, Md .He needed it to house his second large telescope, a $15,000 model with the ability to capture a wider field than his other scope.
The rest of the time she is at her cedar-shingled Bridgehampton home, constructed from a 1920s Sears, Roebuck kit.
Past the reception desk (constructed from a reclaimed aeroplane wing, obviously), pool tables, coffee machines, beach huts and video-conferencing room kitted out with aeroplane seats, I arrive at Burj Al Arab, a room named after the famous Dubai hotel.
A paired-end library was constructed from the cDNA synthesized using a Genomic Sample Prep Kit (Illumina).
He constructed it from a $49.99 kit after reading about them in a magazine article by Mr. Moog, who was selling theremin kits to finance his graduate school education in physics at Cornell University.
But for £50, enthusiasts adept at wielding a soldering iron could construct a Sinclair ZX81 from a kit.
A genome walker library was constructed from A. vastus genomic DNA using the GenomeWalker Kit (BD Biosciences) and restriction enzymes DraI, EcoRV, PvuII, SmaI, and StuI.
The normalized cDNA library was constructed from 200 ng of the poly(A) + mRNA using a TRIMMER cDNA Normalization Kit (Evrogen, Moscow, Russia) and SMART cDNA Library Construction Kit (Takara Bio).
A RACE cDNA was constructed from total RNA using the 'SMARTer™ RACE cDNA Amplification Kit' according to the manufacturer's instructions (Clontech, Mountain View, USA).
Transcriptome libraries were constructed from rRNA-depleted (Ribo-Zero Plant Leaf rRNA Removal Kit; Epicentre) total RNA using a TruSeq mRNA stranded sample preparation kit (Illumina) and sequenced using a MiSeq instrument, which yielded read lengths of 259 + 259.
