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Integration plasmids were constructed for transformation with up to three copies of the crtYB gene.
In total, four transformation vectors, designated pLSP15 (PHB) and pLSP20 (PHBV), and pLSP13 (PHB) and pLSP23 (PHBV), were constructed for transformation in Arabidopsis thaliana and oil palm, respectively.
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In this paper, an analytical solution to the modified MSE MMSEE) in terms of Taylor series is constructed for wave transformation by a trench or breakwater with general trapezoidal shape.
The vector pDELhpr2 was constructed for the transformation of QM9414: A 1113 bp fragment of the 3′-flanking region of hpr2 was amplified by PCR using primers DELHPR23F and DELHPR23R, digested with XhoI -Acc65I and cloned into the XhoI -Acc65I sites of pBSXH (Schmoll et al., 2005).
Binary vectors were constructed for Agrobacterium-mediated transformation using the MultiSite Gateway LR clonase reaction (Invitrogen), as described previously (Wakasa et al., [2006]).
For example, an HTML template was built to visualize the data, whereas an OpenRefine export template was constructed for the automated transformation of tabular data held in standard spreadsheet into JSON objects.
(A) Structure of T-DNA region in the expression construct for rice transformation.
Note that the same terminator $2 is used for all the substrings of row i: and. Figure 11 shows the generalized suffix tree T right constructed for the matrix after alphabet transformation in Figure 9 together with the four maximal 1-CCC-Biclusters (B1, B2, B3 and B4), identified by four valid models, when e = 1, q r = 3, q c = 2 and the algorithm "jumps over" missing values.
The RB gene construct used for transformation was the same as previously published [17].
The alternate use of end-linkers from the two sets pELB-n and pELR-n for successive cloning steps allows the process to be repeated indefinitely from the stand point of the cloning strategy (Fig. 6), but will ultimately be limited by construct size for transformation in standard bacterial hosts such as E. coli.
The nature of the construct used for transformation can be a source for phenotypic alterations.
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