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After filter and quality control, all clean reads were assembled using the PacBio HGAP Analysis 2.0 and one circular chromosome with 0 gap was constructed for further analysis.
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Means of summated multiple item measures were computed and used to represent model constructs for further analysis.
A reliability check was undertaken during this process in order to investigate if we could use the constructs for further analysis.
Additional duf enhancer-deletion reporter constructs for further analysis (results not discussed) are in Supplementary data Figure S1.
Time series of the RRI and the SBPs were constructed and saved for further analysis.
In this study, we utilised bioinformatics analysis to identify the putative Brn-3b promoter and cloned this regulatory region into a reporter construct for further experimental analysis.
At day 14 after surgery experiments were terminated and constructs explanted for further analysis.
Cycle 3, however, showed maximal enrichments for most clones, since the majority of the 75 scFv constructs, selected for further analysis, dropped their relative representation during selection cycle 4.
Cells stably transfected to overexpress wild-type and NLS-mutated PTHrP exhibited an increase in PTHrP expression between 1000- and 10 000-fold compared with vector-control cells and two individual clones for each cell line and construct were selected for further analysis.
We collected 752,841 insect ESTs from the dbEST [ 36] and constructed a local database for further analysis.
All these data are primary materials required to construct the integrative network for further analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com