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We constructed expression systems for recombinant human annexin A2 (rhA2) using Pichia pastoris.
The newly constructed expression vector pSAK was designed for integration into the α β tubulin locus, which is tandemly arranged in the T. congolense genome.
We constructed expression vectors for different NF90ctv protein domains (Figure 1A), co-transfected HeLa cells with the Gag-RRE vector (pCMVGag2RRE), and assessed pr55Gag expression by Western blots.
The newly constructed expression vector was named pET cg10062).
ZM W constructed expression vectors for some of the genes.
Next, we constructed expression plasmids containing N-terminal coding portions or the complete gvpC gene.
NT constructed expression profiles of breast cancer and participated in the screening of candidate genes.
We constructed expression vectors for constitutively active mesotrypsin, caspase-14, kallikrein-related peptidase-5 (KLK5), and KLK7.
We constructed expression plasmids using pcDNA3.0 vector (Invitrogen, Carlsbad, CA, USA), then transfected the constructed expression plasmids into 293 T cells using Lipofectamine 2000 (Invitrogen), and finally collected cells in TRIzol (Invitrogen) 48 h after transfection.
The data confirm that the constructed expression vector provided multi-copy integration into the genome of S. cerevisiae.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com