To validate these target sites, the 3′-UTR of human MXD1 was amplified and inserted in both orientations downstream of the luciferase gene in the pGL3-control vector, generating sense (S) and antisense (AS) constructs, collectively referred to as Luc-1 in Figure 5a.
Any misfitted item was deleted because it did not align with the construct captured collectively by the other items.
"Black-Jewish relations," Jeffrey Melnick argues, has mostly been a way for American Jews to talk about their ambivalent racial status, a narrative collectively constructed at critical moments, when particular conflicts demand an explanation.
A cognitively oriented discourse analysis then shows how the use of a small set of cognitive relational primitives in the underlying ontology opens possibilities for offering users advanced forms of computational service for analysing collectively constructed argumentation networks.
What if artistic sensitivities… currently in deep conflict with the world we have collectively constructed… available to all but latent in most, could act in a direct, unmediated way upon the world – without passing through the medium of the canvas, the carving stone, the text or performer?
"Economic activity is driven by meaning maintaining equal possibilities for the good life while lowering emissions dramatically rather than profit, and the meaning is politically, collectively constructed.
As with the FGDs, our observations allowed us to explore interactions between people and within social contexts where meaning is collectively constructed.
The idea of Activity model is derived from the Activity Theory (19), which sees human behaviour as being part of complex and continuously collectively constructed systems of activity.
Using a social constructionist perspective, we sought to map out features of the collectively constructed representational field which shaped both nurses' and carers' understandings and actions, rather than seeking to document attitudes conceived of as properties of individuals (Gaskell, 2001).
