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For the construction of pTCRKnlux, an intermediate plasmid was constructed by inserting SalI-NruI fragment of pSLlux (luxCDABE genes under the lac promoter) into SalI-StuI-digested pSL1190.
The eukaryotic expression plasmid pcDNA-L was constructed by inserting mouse LIGHT gene into the vector pcDNA3.1.
It is constructed by inserting one tensegrity segment into another.
A triangular prism is constructed by inserting a third tensegrity segment "C" into the previously seen square.
Strain GY02 was constructed by inserting BY4741 PDR5 gene (BPDR5) into the PDR5 locus of GY01.
The UP-plasmid was constructed by inserting the ULTRA upstream of the 2 kb segment.
HIF-1α-expressing vector was constructed by inserting HIF-1α cDNA into pcDNA3.1 vector.
pAAV5neo-EGFP was constructed by inserting EGFP between the CMV promoter and BGH polyA in pAAV5neo.
A pSC35-DsRed2 was constructed by inserting the SC35 PCR product before the DsRed2 coding region.
The plasmid encoding CXCR4CFP was constructed by inserting the PCR product of CXCR4 into the pECFP-N1 vector.
A complemented strain (ΔpA3731comp) was constructed by inserting a wild-type copy of pA3731 in the pA3731 mutant strain.
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