Sentence examples for constructed by addition from inspiring English sources

For example, compounds 32– 35 were constructed by addition of 2 equiv of organomagnesium reagents, whereas compounds 36– 40 and 45– 49 arose by sequential addition of organolithium reagents and Grignard reagents (Table 2).

For Mdj1 protein purification, plasmid pBAD22A MDJ1Δ55 HIS 6 was constructed by addition of PCR generated fragments encoding 6xHis-tag at the 3′ of MDJ1Δ55 (which lacks the codons for the mitochondrial targeting sequence, residues 1 to 55) into the pBAD22A vector [17].

Targets contrasting in intensity and polarization were constructed by addition of a further two concentric rings (with the same diameters as the polarization filters) of 0.3 and 0.9 neutral-density filters (209 and 211 Lee Filters) on top of the polarizing filters.

An engineered horseradish peroxidase isozyme C (HRP C) gene was constructed by the addition of a 6xArg fusion tail to 6xHis HRP C by the PCR strategy.

Recombinant forms have been constructed by successive addition of restricted mutations and one deletion outside the T cell epitope-containing regions.

The scFv of Nor155 was constructed by an addition of (Gly4Ser)3 as a linker between VH and VL regions and subcloned into pPICZαA, an expression vector of Pichia pastoris.

A ribbon lattice, consisting of repetitions of the barcode pattern with expected periodicity, was also constructed by the addition of sticky ends.

Comparison of the patterns observed at intervals of up to three weeks show that this part of the brain is gradually constructed by the addition of new glomeruli to a persisting population.

It is constructed by polarised addition of new subunits at the distal end of the growing structure, in a process governed by intraflagellar transport (IFT) [4], [5].

Myr-ZAP-70 was constructed by the addition of the avian src myristoylation sequence (MGSSKSKPK) at the N terminus of ZAP-70 cDNA[11] (kindly provided by Dr. Andrey Shaw, Washington University).

The initial tree topology was constructed by random addition, the stopgen and stoptime parameters were both set to 10,000,000 and search termination settings were set at default values.