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MR IF plasmids containing portions of TGFBR2 or ACVR2 were constructed as positive controls for EGFP expression (Fig. 1).
Similarly, a full-length CMV promoter reporter was constructed as positive control to validate experimental parameters such as transfection conditions and efficiencies.
Similar(57)
A known enhancer, RER for Rhodopsin gene (Nie et al., 1996), coupled with βGP-GFP was constructed as a positive control.
The original human GBGT1 gene construct, as well as several others, did not produce any Forssman antigen-positive cells (0%), whereas a decrease was observed with several other constructs.
The solution is constructed as the limit of positive solutions in bounded domains.
To determine the percentage of samples (tissues) with positive FRET, we considered any ROI with FRETeff ≥10% after scanning regions where the two constructs co-localised as positive.
The SV40 promoter reporter construct served as positive control.
Untransformed BL21 DE3) cells were used as negative control and the EcNhaA constructs as positive controls.
Data were constructed as numerous MPs depend on marker presentation (positive or negative) and determination of MP populations.
The Negative-Positive (NP) Network was constructed as described by [ 26].
Molecular subtypes were constructed as follows: Luminal A, Luminal B, Triple Negative, and HER2 positive.
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