22 independent lines were obtained that harbored the 35S TM8 construct as determined by PCR analyses carried out with genomic DNA extracted from leaves (see Methods - generation of transgenic plants).
Combined administration of S. enterica serovar Typhimurium expressing chIFN-α and chIL-18 showed markedly enhanced protection compared to single administration of the construct, as determined by mortality, clinical severity, and feed and water intake.
The similar amounts of SHBG mRNA in all LNCaP cells transfected with the different SHBG-pDsRed constructs, as determined by SHBG Ex2-Ex8 RT-PCR (Figure 6A), rules out the possibility that variations in SHBG protein levels were due to differences in transcriptional regulation or transfection efficiency.
The following sections summarise the models/tools by name, aims, and constructs, as determined from assessing the 8 articles and additional original publications where applicable, and the findings are presented as written narratives and in tabular format (Table 2).
This sample size based on the minimum number of participants advised as needed to test the internal validity of the constructs as determined by the developers of the TPB tool [ 31] and considered feasible by the EHOs.
ClustalW [93] multiple protein alignments were used to construct phylogenetic trees as determined by the Neighbor Joining method with bootstrap re-sampling (detailed in the legends for Figures 1), using MEGA3.1 [94].
The right to development conceives of health as socially constructed and determined and emphasises collective responsibilities of the individual, state and (crucially) the international community "to co-operate with each other in ensuring development…to promote a new international economic order based on sovereign equality, interdependence [and] mutual interest" (58).
Inhibition of STAT3 activity by antisense or siRNA downregulation or expression of a dominant-negative construct limited cell motility as determined by an in vitro wound healing assay and invasiveness through a extracellular matrix barrier.
We have added a western blot inset to the new Figure 5B; the data came from light micrographs where we are certain that the construct is actually expressed, as determined by Myc-antibody staining, all quantified by CellProfiler to be sure that we are looking at cells with comparable levels of myc-construct expression.
