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Paraffin blocks of RL95 and HEC-1-A were constructed and processed for PTEN immunostaining as described above.
In order to examine the efficiency of detecting the ACH mutation, different ratios of mutant to wild-type were constructed and processed by the assay (Pre-BEA PCR and BEA using the same conditions as for the testis DNA).
In constructing the BAC library, nuclear DNA was isolated from the growing tips of S. mollendorffii sporophytes, partially digested with Hind III and the library constructed and processed essentially according to Luo and Wing [ 11] as described in the Methods section.
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This bounding region, denoted spiral fat arc (SFA) is simple to construct and process, and shows a cubic approximation order to a given spiral curve.
Based on these discovered genetic, epigenetic, and expression alternations, models of tumor progression have been constructed, and the process of tumor progression and metastasis has been studied.
The new multiple sequence alignment is provided as input to SCI-PHY, subfamily HMMs are constructed, and the process is iterated until no new sequences match inclusion criteria, or the number of sequences or iterations reaches a pre-defined cutoff.
Embryos (F1) from the founders showing the best representative expression pattern for each construct were selected and processed for WISH, as described above.
This construct was expressed and processed in DU145 cells in a manner identical to that of wild-type APP695 (Fig. 4B).
*MIU = Minor Injuries Unit, WIC = Walk in Clinic Based on the agreed rule set, a computer algorithm was constructed and survey responses were processed.
The building has been constructed and actually in process of monitoring.
NURBS surfaces modelling the hull of the vessel are constructed and the fairing process adapted to maintain certain ship characteristics is described.
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