Sentence examples for constructed and normalized from inspiring English sources

Double-stranded cDNA was constructed and normalized from these six isolated, purified RNA samples using the Trimmer-Direct Normalization Kit (Evrogen, Moscow, Russia) in conjunction with the Creator SMART cDNA Library Construction Kit (Clontech, Mountain View, CA).

For each tweet t w i, i∈{1,…,m}, from the dataset D, a BoW vector v i of term frequencies T F i (t) for each term t in t w i is constructed and normalized.

Control-FREEC takes as an input aligned reads, then constructs and normalizes the copy number profile, constructs the B-allele frequency (BAF) profile, segments both profiles, ascribes the genotype status to each segment using both copy number and allelic frequency information, then annotates genomic alterations.

Then, an eight-dimensional feature vector can be constructed by calculating and normalizing the energy of each band.

Relative mRNA was quantified using standard curves constructed from fetal brain RNA and normalized to total cellular mRNA content using HPRT as a neural housekeeping gene.

GAG content in the media was analyzed (n = 3) by using the dimethylmethylene blue assay and normalized per construct.

Then, the four microbial abundance matrixes and four environment factor matrixes of spring, autumn, fall, and winter seasons were constructed by normalizing every OTU and environment factor vector with zero-mean normalization method.

An initial cDNA library was constructed using the SMART cDNA Synthesis Kit (BD Clonetech, Mountain View, CA) and normalized using the Trimmer-Direct Kit (Evrogen, Moscow).

The value for binding of wild type SU-Fc to cells was arbitrarily set to 100%, and the binding data for specifically constructed mutants were normalized to this value.

Start and normalized directionally cloned (3' NotI-5'EcoR1) cDNA libraries were constructed as previously described [ 47].

Firefly was divided by Renilla activity and normalized to the negative control for each construct.