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Series of one FAC repeated three times, separated by three seconds, were constructed and normalised to ensure all series were broadcast at a consistent amplitude.
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DS constructed normalised and non-normalised libraries.
PDE activities were then normalised for expression of construct, and the data were normalised to untreated (NT) wild type PDE4D7 activity.
The libraries were constructed, normalised and subtracted following the protocol of Soares [ 42] with minor modifications.
In order to greatly expand the genomic resources available for B. terrestris we constructed a normalised expressed sequence tag (EST) library from thorax and abdomen tissue of workers.
We constructed a normalised EST library of A. minutum to search for PSP toxin-related genes in this dinoflagellate.
Stepwise multiple-linear regression was performed on the normalised terrain attributes and on the principal components constructed from the normalised terrain attributes to avoid multi-collinearity.
The activity of each Prod 1 promoter construct was normalised to the activity of the internal Renilla control and expressed as the ratio (Firefly/Renilla).
For each data segment, construct a normalised 2D histogram of RR(n) vs RR(n + 1) and its contour map.
Samples from the test or other data set are assessed, using an n-biomarker diagnostic model, for their stem cell state by constructing a normalised vector of the expression levels of the n-biomarker genes in the sample to be tested.
Total RNA was isolated from hippocampi of 5 adult marmosets, both males and females ranging from 3 to 10 years in age, After checking the quality of the isolated RNA it was pooled and used to construct a custom normalised cDNA library in pCMVSport6.1 (Invitrogen, Carlsbad, USA).
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