Sentence examples for constructed and isolated from inspiring English sources

Moreover, a ClosTron-based pMTL007-adc containing the modified intron targeting the adc gene (encoding acetoacetate decarboxylase) was constructed and isolated from E. coli JM109 without methylation.

Wild type MtIPMS and all variants reported here were constructed and isolated as previously described.

We stably transfected BaF3 cells with either the WT- or the K762R/G-CSFR construct and isolated by limited dilution three independent clones each expressing similar levels of the WT or the K762R/G-CSFR, removed them from IL-3-containing media, washed them, then treated them with varying concentrations of G-CSF for a period of 72 h.

Agrobacteria carrying Cauliflower mosaic virus (CaMV) 35S promoter-based constructs and isolated from the injected mice retained their capacity to promote green fluorescent protein (GFP) synthesis in Nicotiana benthamiana leaves.

We further mapped the interaction of Np95 with Dnmt3a/b through co-immunoprecipitation of deletion constructs and isolated domains transiently expressed in HEK293T cells (supplementary Fig S2 online).

HeLa cells were transfected with the GDAP1-FLAG construct and mitochondria isolated after 24 hours, digested with protease K in the presence or absence of the detergent digitonin, followed by Western blot analysis (Fig. 1A) [23].

Nowadays, several allergen-specific IgE and IgG antibody display libraries have been constructed and recombinant antibodies isolated e.g. against pollen, cow's milk and latex allergens [ 30, 31, 33- 36].

Four additional proteins were constructed and analyzed to isolate the effects of helix dipole and N-capping interactions in each helix.

An E. pulchra head cDNA library from circadian and tidal samples was also constructed and used to isolate the full-length Eurydice period (Epper).

A quality assessment list was constructed and used to isolate papers with high quality methodological criteria (scores of 13 or above, HQMC).

A mevalonate biosensor was constructed and we succeeded in isolating a mutated strain (Q49) with a 60% increase in mevalonate concentration (an acetyl-CoA derived product) following sensor-bacetyl-CoA derivedut screening of a QscR transcriproduct regulator library.