Sentence examples for constructed and introduced from inspiring English sources
The plasmid pSac-bglB was constructed and introduced into Z.
Finally, plasmid pGAPZA-WZ (5046 bp) was designed, constructed and introduced into the yeast P. pastoris to produce astaxanthin (Figure 2).
A biosynthetic pathway for the production of 4-hydroxybutyryl-CoA was also constructed and introduced into the poly-3-hydroxybutyrate-producing strain.
For this purpose, different synthetic isopropanol operons were constructed and introduced on plasmids in a butyrate minus mutant strain (C. acetobutylicum ATCC 824 Δcac15ΔuppΔbuk).
A new deformation model considering both the external (mainly climatic) and internal factors (such as tectonic activities and thermal character of frozen soil) is constructed and introduced into the SBAS-InSAR to estimate the surface deformation over permafrost.
A vector with hydrogenase genes (hoxEFUYH) from Synechocystis sp. PCC 6803 under an alkB promoter was constructed, and introduced into Escherichia coli DH5α to alter the hydrogen metabolism with glucose as the sole carbon source.
The AahII scorpion toxin gene was amplified by PCR from the pEU-AahII-GST construct and introduced into pET28a vector between BamHI and HindIII sites.
To explore the physiological role of OsNAP in leaf senescence, we generated an OsNAP RNAi construct and introduced it into Zhonghua 11. 10 single-copy insertion lines were verified by Southern blot analysis (Additional file 1: Figure S5).
Where the f-block is concerned, there are no examples of actinide cyclo-P5 derivatives,[ 7] and only two examples incorporating lanthanide ions are known,[ 8] which notably both contain the d-block fragments used to construct and introduce the cyclo -P5 unit.
To address this issue, several databases that focus on specific topics have been constructed, and are introduced as follows.
A DNA fragment carrying the cKJUMPS expression cassette flanked with piggyBac transposon terminal repeats was then constructed (TR_cKJUMPS) and introduced into an Ura− mutant, and Ura+ transformants were isolated.
