Sentence examples for constructed and digested from inspiring English sources

Homodimeric ey-TALEN expression vectors pT3TS- ey-TALEN-left and pT3TS- ey-TALEN-right, were constructed and digested with XbaI and BsaBI endonucleases.

After material testing, half of the construct was dried and digested in proteinase K solution overnight at 56 °C, as described previously [ 35], and the other half was preserved for histology (see below).

One correct clone for each of the constructs was selected and digested from the pCR-Blunt II-TOPO vector via double digest using BamHI and HindIII restriction enzymes (Fermentas) according to manufacturer specifications.

This construct and pERL02 were digested with both BamHI and NcoI and then gel purified to extract the 2-kb VTI13 promoter fragment from pERL03A and the promoterless pERL02 linear plasmid.

Each construct was digested and sequenced in two reactions, one containing the forward primer, the other containing the reverse.

Constructs were lyophilized, homogenized, and digested in a papain solution [100 mM sodium phosphate buffer, 10 mM Na2EDTA, 10 mM l-cysteine, 0.125 mg/mL papain (Worthington)] for 16 h at 60 °C.

pCZ486 contains a deletion only in the drsh-1 coding region and was constructed by digesting pCZ483 with SphI and religating.

The pGL3hres0.7K construct was digested with KpnI and ApaI and the plasmid re-ligated to generate pGL3hres0.34K construct.

The resulting construct was digested with NdeI and HindIII and the excised fragment was cloned between the same sites of pET28a+ (Novagen) to generate pOpp102.

The construct was digested with NotI and SalI and the linear transgene cassette was microinjected into the pronuclei of fertilized CD-1 oocytes.

The construct was digested with BsaI and AatII and ligated with a 589 bp siaA fragment (nt. 571 to 1160), amplified using primers NG1306 (5'-GCAGACGTCGCCTAGTGCAAATGGGAGAA-3') and NG1307 (5'-gcaggtctcatctgtgctggtgcgagtatc).