Because our goal was to assess the biological signals from transcripts that could be compared directly with their corresponding proteins, and vice versa, we constructed a "paired" data set, consisting of theoretical transcript parents each paired to a single protein product (522 pairs in the ACC, 499 in the CN once one outlier was removed from the analysis in each region [see below]).
For every pair of proteins in the complex, we then constructed a paired multiple sequence alignment ('Materials and methods').
Gober had brought with him an assistant and a welder, who had constructed a pair of aluminum T-bars that would offer additional support to the work when it was remounted on a wall.
And up on the civic centre plinth, overlooking the plaza and the city beyond, Bureau des Mésarchitectures have constructed a pair of swings, called Double Happiness (2009), raised on a 10-foot-tall platform.
We have constructed a pair of Quasi-Sheet dipole magnets, each an iron box enclosing Nd Fe B blocks that produce a 0.486 T field adjustable to ±2% and uniform to better than ± 0.05% in a 500×250×20 mm3 working region.
With insect flight it fails miserably". To find out what other forces insects take advantage of, he and his coworkers constructed a pair of 25-centimeter-long (10 inches) Plexiglas wings, modeled after the wings of the fruit fly Drosophila melanogaster, and immersed them in a tank of mineral oil.
Since flotillin-1 and flotillin-2 were reported to form the hetero oligomer (Babuke et al., 2009), we constructed a pair of positive control BiFC constructs, consisting of flotillin-1-NsfGFP and flotillin-2-CsfGFP, and co-transfected them into HEK-293 cells.
The Fore River Shipbuilding Company and Bath Iron Works built one ship each, while William Cramp and New York Shipbuilding each constructed a pair of Tucker destroyers.
We cultivated C. velia CCAP 1602/1, isolated polyA mRNA and constructed a paired-end cDNA library for Illumina sequencing.
To detect a distance between these strands we constructed a pair of mutants containing residue 91, which points outside the core (see above), versus residues 75 and 76.
For each new sgRNA vector to be constructed, a pair of oligonucleotides are synthesized, annealed, and then inserted into the digested vector by Gibson assembly.
