Sentence examples for constructed a library from inspiring English sources

As a model we constructed a library of octapeptides and showed that the library is amenable to selection on cos-7 cells.

Subsequently, we constructed a library containing 6.8 × 1010 unique members, in which these 24 positions were diversified with a degenerate codon that encodes for 6 aa that are prevalent in protein interaction sites.

To overcome this limitation, we constructed a library of FHA1 variants by mutagenic PCR and isolated functional variants after three rounds of affinity selection with its pT peptide ligand.

We constructed a library of PDZ domains by randomizing residues in direct contact with position 0 and in a loop that is close to but does not contact position 0. We used phage display to select for PDZ variants that bind to 19 peptide ligands differing only at position 0. To verify that each obtained PDZ domain exhibited the correct binding specificity, we selected peptide ligands for each domain.

To this end, we constructed a library of size-fractionated RNAs from S. japonicum adult worms and analyzed 227 RNA sequences from a total of 106 insert-containing clones randomly selected from the library.

To achieve a better understanding of the antigenic repertoire of the CD8 and CD4 T-cell responses against KSHV, we constructed a library of lentiviral expression vectors each coding for one of 31 individual KSHV open reading frames (ORFs).

Illumina MiSeq library construction Approximately 5 μg cpDNA was used to construct a library containing a 400 bp insert size.

This suggests it would be feasible to construct a library of DNA signatures linked with English family names.

The resulting high quality cDNAs were used to construct a library for paired-end sequencing.

Using 33 commercially available monomers it is theoretically possible to construct a library of over 40,000 distinct polymer compositions.

Utilizing this platform, we construct a library of orthogonal synthetic transcription factors (sTFs) and use these to wire synthetic transcriptional circuits in yeast.