Sentence examples for construct with the helper from inspiring English sources
When the cultures attained an OD of 0.6 to 0.8, we centrifuged them (1,125 g, 4°C for 5 min), resuspended the pellets in an equimolar mix (5 mM) of MgCl2 and MES and prepared a 1 1 mix of each construct with the helper strain pBINTRA.
We transfected one pair of human lung cancer cell lines: 95C and 95D cells with the miR-101 vector and a vector encoding the antibiotic marker, we selected the antibiotic resistant colonies from the transfected cells; 2. We used the lentiviral construct with the viral helper to infect one human GBM cell line, U87MGD cells, and collected the cells at 48 72 h after infection.
AAV vector was generated by triple transfection of the AAV ITR flanked construct with the packaging plasmid pAAV2/8 and the helper plasmid pdF6 as previously described [20], [21].
A plasmid, harboring a Tol2 construct with the gene expression cassette flanked by Tol2 cis-sequences, and a helper plasmid, containing the transposase-coding sequence driven by a constitutive promoter, are simultaneously introduced into vertebrate cells.
The NeoGag construct, together with the pCasper helper, was transfected into Sf21 cells.
For retroviral production, HEK-293T cells were transfected with the required retroviral construct along with helper plasmids.
With a self-eliminating helper plasmid, we successfully removed the target plasmid pCNB1, along with the helper plasmid itself, in a single step at an efficiency of 64%.
The sender binds with the helper until the associated cooperative transmission fails or the sender finds that there is a helper with better link quality.
The maze was constructed with gray plexiglass.
