Sentence examples for construct were identified from inspiring English sources

The targeting constructs were electroporated into 129 ES cells and 4 independent homologous recombinant clones for each construct were identified.

MES cells carrying a single copy of the SIRT1STOP construct were identified by resistance to the antibiotic marker hygromycin and Southern blotting.

Transformants were selected on minimal medium supplemented with p-aminobenzoic acid and strains with a single ectopic copy of the niiA(p)::dmtA+::argB+ fusion construct were identified by Southern hybridization.

Clones resistant to the neomycin analog G418 were selected and screened by PCR using Expand Long Template PCR System (Roche) and two primers: RecF (5'-GGCATAGGCTCCTGAGGC) and T (5'-GGTCCACGACCCAAGCTG). ES cells that had undergone homologous recombination with the targeting construct were identified by a 2456 bp product.

Transgenic plants containing both the reporter and an effector construct were identified through antibiotic resistance screens.

(B ) Off-target genes for the LYS1-amiRNA construct were identified using the Web microRNA Designer (WMD; http://wmd.weigelworld.org ).org

A hydrogen bonded dimer molecular assembly or supramolecular construct was identified in all the crystal structures examined along with a further four 1D supramolecular constructs which were common to at least two of the family of structures studied.

Construct validity was supported by factor analysis and a single underlying construct was identified.

By testing four hairpins against either aldoaa or kif22, a targeting construct was identified for each gene.

The fraction containing the Tg construct was identified by pulsed-field gene electrophoresis (FIGE Mapper, Bio-Rad).

Once the markers for which haplotypes were to be constructed were identified for each gene, then Haploview was re-run to identify the likely haplotypes and determine their relative frequencies.