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The construct was verified by DNA sequencing (GenScript, Nanjing, China).
The entire construct was verified by sequencing.
This construct was verified by restriction digest.
The resulting expression construct was verified by restriction digest.
The sequence of the construct was verified by DNA sequencing.
The final construct was verified by dideoxy chain termination sequencing.
The entire fusion construct was verified by sequencing.
The fabricated construct was verified by DNA sequencing.
Single integration of the deletion construct was verified by Southern blot (data not shown).
The integrity of the pHluorin-APP construct was verified by sequencing.
The resulting construct was verified by sequencing (GeneWiz, South Plainfield, NJ).
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