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Following the cloning, this construct was transformed into the rice genome by Agrobacterium-mediated transformation.
The construct was transformed into tpx mutant by electroporation and gentamicin resistant transformants were selected.
The construct was transformed into the gi-2 mutant background and kanamycin-resistant transformants selected.
This construct was transformed into MA70.15 (ΔkusA, pyrG −, and amdS + ), and transformants were purified by repeated streaking of conidia on media without uridine.
The construct was transformed into pkac- cells and G418 resistant cells were selected as described above.
Alternatively, the Tth construct was transformed into competent ClearColi® BL21 (DE3) cells (Lucigen, BioCat GmbH, Heidelberg, Germany).
This construct was transformed into Escherichia coli cells harboring the genes for phytoene production.
The construct was transformed into Escherichia coli, and transformed cells were stored after being freeze-dried in the presence of sucrose.
The construct was transformed into Agrobacterium tumefaciens strain EHA105.
This construct was transformed into Escherichia coli DH5α cells.
This overexpression construct was transformed into japonica varieties Nipponbare and Wuyunjing 7.
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CEO of Professional Science Editing for Scientists @ prosciediting.com