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After TA cloning, the construct was sequenced to confirm no errors have been introduced.
The construct was sequenced.
The pBAV1KT5-gfp construct was sequenced.
Each construct was sequenced to ensure no undesired mutations introduced in the process of cloning.
The construct was sequenced to ensure that the sequence and orientation were correct.
Near full-length genome of the construct was sequenced using primer walking method described previously [6].
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All construct were sequenced.
After identification of recombinant clones, three inserts from each construct were sequenced (Genomex).
All DNA constructs were sequenced using an automated DNA sequencer (Perkin Elmer).
All the DNA constructs were sequenced by GENEWIZ (Suzhou, China).
All constructs were sequenced.
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