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The pELMO construct was propagated on One Shot ccdB survival cells (Invitrogen).
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The chimeric constructs were propagated in E. coli DH5∝.
All constructs were propagated in E.coli DB3.1λpir [ 52].
Constructs were propagated in E. coli, followed by electroporation into S. aureus RN4220 133).
The resulting plasmid constructs were propagated by standard procedures and purified using a Maxi-Prep Plasmid Kit (Qiagen).
Escherichia coli cells were transformed with the pertinent IL-60 construct and propagated under ampicillin selection, and the construct was extracted by standard procedures [ 19].
After the virus was propagated in cell culture, overlapping fragments were sequenced using degenerate primers and were assembled to construct the complete genome sequence.
From transformation of thousands of explants, 8 to 10 transgenic potato lines for each amiRNA construct were selected and propagated for molecular genetic screening and analysis (Table 3).
The construct was sequenced.
LCMV Armstrong was propagated on BHK cells.
C. reflexa was propagated vegetatively throughout.
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