Sentence examples for construct was performed using from inspiring English sources
Stable transfection of HEK293 cells (ATCC, cell line CRL-1573) withethe myc-tagged Ctr1 construct was performed using FuGENE® HD (Roche) following the manufacturer's instructions.
The SH3 deletion construct was performed using the forward primer lst-4_dSH3_AscI using pJA17 as a template, followed by cloning into pLN180 to generate pJA47.
Transient introduction of the pARR-2PB-dsRED2skl reporter construct was performed using 6.0 µg reporter plasmid and 6.0 µg carrier DNA (BlueScript, Stratagene, San Diego, CA) using Transfectin (Biorad) following standard protocols.
Transient introduction of the pARR-2PB-dsRED2skl reporter construct was performed using 6.0 µg reporter plasmid and 6.0 µg carrier DNA (BlueScript, Stratagene, San Diego, CA) using Transfectin following standard protocols.
Mutagenesis of the construct was performed using the QuikChange Site-Directed Mutagenesis system (Stratagene).
Site-directed mutagenesis of promoter constructs was performed using QuikChange kit (Stratagene).
Transfection of ezrin expression constructs was performed using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA) according to the manufacturer instructions.
Transient transfection of plasmid constructs was performed using Lipofactamine 2000 (Invitrogen) in HEK293T cells, and siRNAs were transfected using jetPRIME (Polyplus transfection) in HLE cells.
Transformation of WT Col-0 and ft-10 mutant plants with Agrobacterium strain EHA105 carrying the recombinant constructs was performed using the floral dip method [ 49].
Transfection of constructs was performed using Lipofectamine 2000 and RNAi-max (Invitrogen) for cDNA or siRNA, respectively, in accordance with manufacturer's instructions.
Transformation of WT Col-0 and co mutant plants with Agrobacterium bacteria carrying recombinant constructs was performed using the floral dip method [ 81, 82].
