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The construct was maintained in TOP10 F' Escherichia coli cells (Invitrogen, Carlsbad, USA).
It was therefore important that the structural integrity of the 3D construct was maintained throughout the culture period.
pEPito-hCMV/SM22-EGFP-IRES-BSD could only be rescued from genomic DNA of the stably selected TE-671 cells, indicating that this construct was maintained here as an epsiome.
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Mice expressing the chimeric (human/mouse) DRB1*0101 construct were maintained in our onsite pathogen-free facility.
Mice expressing the chimeric (human/mouse) DRB1*0101 construct were maintained in our onsite facility.
Cells stably carrying each shRNA construct were maintained in culture medium containing blasticidin at 5 μg/ml.
Steadily transfected cells expressing APP construct were maintained in G418 at a final concentration of 600 μg/ml.
Fixation strength of the construct is maintained, since engagement of the screw by the SLE is independent of the bone quality.
One possibility to bypass this disadvantage is the use of autonomously replicating vectors, where the complementing gene construct is maintained extrachromosomally.
These tissue constructs are maintained by their own defined blood supply and are called flaps.
The cell-substrate constructs were maintained in an incubator (37 °C and 5% CO2) for further examination.
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