Sentence examples for construct was ligated from inspiring English sources

This construct was ligated into the pEX18Gm vector between the HindIII and the KpnI sites.

Briefly, the PCR amplified construct was ligated into the pET-29a vector (Novagen) between the NdeI and XhoI sites.

The construct was ligated using T4 ligase and checked by XcmI digestion and sequencing of the promoter region.

Each synthesized oligonucleotide construct was ligated to the T7 vector arm.

This deletion construct was ligated with pDS3.0 and then transferred into E. coli S17-1/λpir by electroporation.

The DNA encoding the full-length construct was ligated into the pSin lentiviral backbone (Addgene, Cambridge, MA, USA) to create the pSin-chA21-28Z pSin-chA21-28Z pSin-chA21-28Z

All promoter constructs were ligated into the pGL3-basic luciferase vector (Promega, Madison, WI).

HOIP cDNA constructs were ligated into pOPRSV5.neo, an IPTG-inducible expression vector [4].

The C.b.[P], A.s.[P] and C.b.[Im1 12] constructs were ligated into pRAV12 and expressed according to the procedure described above.

Using the HindIII and BamHI sites, the constructs were ligated into the modified MTT1 plasmid, here pK5KOAs.40 and pInv2, respectively and used for biolistic transformation as described by Cassidy-Hanley et al. [15].

Constructs were ligated into pcDNA3.1/Zeo+ or/Neo− (Invitrogen) and authenticated by DNA sequencing.