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The construct was inserted at the attP40 on chromosome 2 (insertion line – Bloomington 25709).
The construct was inserted into E. coli BL21 (DE3) cells through thermal shock.
The optimized construct was inserted into a pFastbac vector for expression in Spodoptera frugiperda (Sf9) cells.
Each scaffold implant construct was inserted into a surgical pocket in triplicate for each assay (using 3 different rats).
This construct was inserted at the SMU.1405 locus, which is well separated from the native SMU.1882 locus [29].
The ECFP reporter construct was inserted in a large BAC, over 200 kbp in length, to insure fidelity of expression by the normal tissue specific regulatory elements.
Similar(35)
Most wheat transformations to date have been achieved using particle bombardment [ 4] in which, in many cases, multiple copies of the construct are inserted, either randomly throughout genomes or within a single insertion site (linked copies).
To address this problem, we introduce here an enhanced reliability Recoverable Block of Function RBFF) system where the blocking construct is inserted in a large artificial intron of a transgene of interest.
However, only background levels are observed when the reporter construct is inserted into euchromatin where the w+ gene is fully expressed.
The results show that the Su TPE) candidates, Hdac1, Su var)3-9 and Hp1c, are all present in abundance when the construct is inserted at the 2L telomere and the w+ gene is silenced (Figure 4).
In Hot1Gt pU-21T 346Card/(Gt pU-21T 346Card mice, the Gt pU-21T 346Cardt is inserted in the first intron of the Hot1 gene.
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