Sentence examples for construct was done using from inspiring English sources
A model construct was done using a forward selection of the variables.
The 3× FLAG-ERβ construct was done by using the same procedure with the following oligonucleotides: S.1 and AS.1 were identical.
This was then used as the DNA template for addition of lysines in all other constructs, which was done using the QuikChange site-directed mutagenesis kit (Stratagene, Santa Clara, CA).
The construct for disruption of PpHsp16.4 was done using the vector pUBW302 [ 37], containing the nptII gene driven by the constitutive 35S promoter and the 3′UTR of the ocs gene.
Phenol quantitation was done using a calibration curve constructed from a fortified matrix in three levels.
Image capture was done using SimplePCI, and figures were constructed with Photoshop.
Absolute quantification was done using plasmid DNA constructs encoding respective genes as standards and normalized to express as gene/actin mRNA level.
Alignment was done using PRANK [73], [74], and the program MEGA4 was used to construct a phylogenetic tree [75].
Content and construct validation were done using a table of specifications filled out by experts including two ethics philosophy professors, and two pediatricians.
Knockdowns in S2 cells were done using the following constructs: an H1 construct obtained from [ 11] and HMGD1 PCR products were obtained from the Drosophila RNAi screening center (DSRC).
The construction of complex spherical waves can also be done using the CGO solutions constructed in [107].
