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In contrast, no binding of domain III construct was detected.
c Expression of Drs-GFP construct was detected at baseline and significantly increased as early as 18 h following infection using Western immunoblot.
The TetO-Myc construct was detected with the following primers: Myc-F 5'-TAGTGAACCGTCAGATCGCCTG-3' and Myc-R 5'-TTTGATGAAGGTCTCGTCGTCC-3' (yielding a 450 bp product).
However, no signal of the GFP-cODC1-TDegF construct was detected after cycloheximide treatment.
If a stop state of the network is found, then a simplification construct was detected successfully.
Expression of hairpin construct was detected in 6 of the lines (cDNA from developing endosperm 20 DAP) by RT qPCR (data not shown).
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No off-targets of this RNAi construct were detected using a web-based search tool (http://www.dkfz.de/signaling/e-rnai3//).
SiRNAs of 23 25 nt homologous to the PPV sequence used in the ihprolC-PP197 construct were detected in transgenic plants before and after inoculation.
Seeing as the abundance of each shRNA expression construct is detected by at least two, in most cases even six different tiling probes, variations resulting from probe sequence biases are minimized.
siRNAs homologous to the 197 bp region of the hairpin construct were detected in inoculated transgenic plants both 2 days after inoculation (Fig. 8, panel A, lanes 2 and 3) and 42 days after inoculation (Fig. 8, panel A, lane 4).
A significant decrease in luciferase activity of both p27-3'UTR constructs was detected in myoblasts compared to myotubes, suggesting that endogenous miR-221/222 are competent for inhibition of p27 mRNA translation in these cells.
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