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The GFP gene construct was designed to express ubiquitously.
An RNA interference construct was designed to target the gene for Pns9 of RGDV, namely Trigger_G9.
In addition an inverted-repeated construct was designed to inactivate DmMRG15 via the RNAi pathway, and RNAi constructs were expressed using both the Tet-on system and Geneswitch system.
In the present study, a human MDR1 promoter reporter gene construct was designed to investigate the reverse effect in which selected activators of the major CYP (1 3) genes were tested for potential inhibition of transcriptional activity of the MDR1 gene.
First, the mini-rRNA gene construct was designed to have a rDNA promoter fused to adjacent 5'ETS segment, which has no known cleavage sites that could recruit processing factors at the transcript level.
An adenovirus-NDRG2 construct was designed to increase the expression levels of NDRG2.
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This construct is designed to bind reversibly to the F-actin cytoskeleton and can be used for PALM imaging due to its photoconversion from a green to a red fluorescent form upon illumination with 405 nm light.
This construct is designed to block PCNA sliding off the DNA in two ways.
The two types of construct were designed to be as similar as possible so that transfection and expression levels would also be similar.
Briefly, the TOP-FLASH construct is designed to measure transcriptional activation mediated by β-catenin and FOP-FLASH is the mutated counterpart of the TOP-FLASH plasmid.
The CASP-19 construct is designed to be separate from its predictors in particular from wealth, social connections and health (Blane et al., 2008; Hyde et al., 2003 Wiggins et al., 2004).
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