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The targeting construct was checked by sequencing and restriction digestion at each stage of its construction.
The construct was checked by sequencing.
The integrity of each construct was checked by nucleotide sequencing.
The FoxO coding sequence within the construct was checked against the NCBI trace archive sequences for H. magnipapillata 105.
The construct was checked by sequencing the entire insert and used as a bait to screen a random-primed human brown adipocyte cDNA library constructed into pP6.
Each construct was checked by sequencing and Western blot analysis and named for the muNS residues that the expressed protein contains (Table 1).
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The integrity of all constructs was checked by sequencing.
Therefore, discriminant validity between these two constructs was checked, and the two constructs were distinct.
All genetic constructs were checked by sequencing (Eurofins, Germany).
The constructs were checked by PCR for gapN or UTR1 genes, digestion with the proper RE combination and sequencing.
Constructs were checked by DNA sequencing.
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