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Moreover, pMCV1.4-p31 pMCV1.4-p31ruct was assayed for molecular adjuvantcity's for a DNA vaccine against SVCV based in the surface antigen of this virus (pAE6-GSVCV).
Repression of a bam-GFP reporter construct was assayed in (8).
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Three independent transgenic lines for each construct were assayed.
The cellulase production levels of transformants from each construct were assayed on β-glucan plates (see below).
PIP2 hydrolytic activity of PLC constructs was assayed as described previously [ 17].
Expression of the constructs was assayed by western blot with anti-V5 antibody (online figure S4).
PIP2 hydrolytic activity of PLCζWT, PLCδ1WT and mutant constructs was assayed as described previously [ 15].
The efficacy of different DNA constructs was assayed in a mouse model, the TC-1 cell line.
These constructs were assayed for interaction with Rab5, first by using the yeast two-hybrid system.
Transgenic flies created using these constructs were assayed for their ability to maternally rescue the tre1sctt germ cell migration defect in the context of developing embryos.
To determine if the efficiency of cryptic transcription correlated with sequence within the DENV2 nt 68 86 region, the presumed −10 element (DENV2 nt 72 79) in pD2-GFP was mutated, and the yields of GFP mRNA produced in E. coli transformed with these plasmid constructs were assayed by quantitative RT-PCR (Table 1).
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