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Finally we describe the general strategy to construct virus deletion mutants that can be engineered to obtain attenuated ASFV strains suitable for vaccine approaches.
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Global mutational analysis by constructing virus gene-deletion mutants classified UL76 as essential [1] or augmenting [4] for viral replication.
Global mutational analysis by constructing virus gene-deletion mutants classified UL77 as essential and UL76 as essential [1] or augmenting [4] for viral replication.
Global mutational analyses of the viral ORF by constructing virus gene-deletion mutants indicates that approximately one-quarter, or 41 ORFs, are required for viral replication in cell culture [4].
To this end we constructed viruses based on VACV strain Western Reserve WRR) lacking up to three intracellular innate immunomodulators (N1, C6 and K7).
To test explicitly whether capsid proteins encoded by V-073-susceptible and V-073-resistant genomes assemble into mixed capsid structures during co-infection, which could be distinguished from separate, homogeneous structures, we constructed viruses whose capsid proteins could be independently identified by antibodies.
Shuttle vector without any insert was used to construct control virus (Ad-co).
Attempts have been made to construct Langat virus which retains its immunogenicity but is less neuroinvasive.
Compared to the neo/gusA selection, EGFP selection provides a direct, fast and convenient way to construct recombinant viruses, indicating the usefulness of the pSPV-EGFP vector.
In this study, we developed an integrated pipeline to construct a virus-host interactome based on protein domain pair, where we hoped to present novel insights that might not be provided in the binary protein interaction networks.
B4 WEEKEND E1-34; E35-44 ESCAPES F1-18 SCIENCE/HEALTH Polio Virus Made in a Tube Scientists said they had constructed a virus from scratch for the first time, synthesizing live polio virus from chemicals and publicly available genetic information.
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