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Compared to the control, progeny derived from the S-cdA construct upon transformation was 0.5% in E. coli with normal repair and replication functions, which increased more than 17-fold with SOS (Table 1).
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Expression and correct localisation of the three constructs upon transformation into Lactobacillus paracasei was verified by Western blot analysis of the supernatant and cell fractions of cultures grown in MRS. The scFv expressed by both the secreted (KKA308) and anchored constructs (KKA307) were found primarily in the expected fraction, the supernatant for KKA308 and the cell fraction for KKA307.
We are constructed upon that.
Her Harry Potter books are constructed upon these values.
We construct the symplectic transformation Ψ.
To obtain the normal form, we construct a symplectic transformation.
The RB gene construct used for transformation was the same as previously published [17].
The nature of the construct used for transformation can be a source for phenotypic alterations.
JM and EP participated in construct cloning, plant transformation and selection of transgenic lines.
The lethality phenotype was specifically due to the RNAi/DUT1 construct as it was not observed upon transformation with an empty vector (RNAi/0).
JGL constructed the plant transformation constructs.
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